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Ir-associated murine alloantigens: demonstration of multiple Ia specificities in H-2 alloantisera after selective
Quantitative absorptions of anti-H-2 alloantisera with increasing concentrations of T cell-derived tumor cells of donor H-2 type has been found to remove antibodies to H-2K region antigens selectivelymsimilar results were obtained after absorptions with thymocytes of the donor strainl In each case residual antibodies reacting with Ir-associated B cell alloantigens (Ia antigens) were unmasked by this procedure. Antisera which initially contained comparable titers of Ia and H-2 activity were depleted of H-2 activity at absorbing cell concentrations which did not appreciably alter the Ia reactivities, indicating that Ia antigens, if expressed on these T cell populations, must have been present at very low concentrations relative to H-2D and H-2K region antigens. Further absorptions with spleen cells from cross-reactive strains demonstrated a multiplicity of shared and unique Ia specificites in these sera. The multiple Ia antigens detected appeared all to be codominantly expressed on the same subpopulation of lymphoid cells.
Quantitative absorptions of anti-H-2 alloantisera with increasing concentrations of T cell-derived tumor cells of donor H-2 type has been found to remove antibodies to H-2K region antigens selectivelymsimilar results were obtained after absorptions with thymocytes of the donor strainl In each case residual antibodies reacting with Ir-associated B cell alloantigens (Ia antigens) were unmasked by this procedure. Antisera which initially contained comparable titers of Ia and H-2 activity were depleted of H-2 activity at absorbing cell concentrations which did not appreciably alter the Ia reactivities, indicating that Ia antigens, if expressed on these T cell populations, must have been present at very low concentrations relative to H-2D and H-2K region antigens. Further absorptions with spleen cells from cross-reactive strains demonstrated a multiplicity of shared and unique Ia specificites in these sera. The multiple Ia antigens detected appeared all to be codominantly expressed on the same subpopulation of lymphoid cells.