Cytophilic attachment of ovine IgG2 to autologous polymorphonuclear leucocytes

Preparations of ovine polymorphs were stained with fluoroscein-conjugated monospecific anti-immunoglobulin sera. Approximately 26% of polymorphs specifically stained with anti-gamma2, 16% with anti-light chain, 24% with anti-Fab and 24% with anti-ovine serum. There was no significant staining of polymorphs with anti-gamma1, anti-mu or anti-alpha. Cytophilic IgG2 could be removed from the cells by incubation at 37 degress and subsequently replaced by incubation in autologous serum at 4 degrees.