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Recent advances in the application of expressed protein ligation to protein engineering
Roseanne M Hofmann1, Tom W Muir
1Laboratory of Synthetic Protein Chemistry, The Rockefeller University, New York, NY 10021, USA.
Current Opinion in Biotechnology
|September 27, 2002
Abstract:
Expressed protein ligation is a technique for joining recombinantly expressed proteins to polypeptides containing biophysical probes, post-translational modifications or unnatural amino acids. Recent advances have expanded the scope of expressed protein ligation and have allowed the approach to be applied to the study of basic biological questions.