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Two-dimensional gel electrophoresis; better than a poke in the ICAT?
Wayne F Patton1, Birte Schulenberg, Thomas H Steinberg
1Proteomics Section, Molecular Probes, Inc., 4849 Pitchford Avenue, Eugene, Oregon 97402, USA. wayne.patton@probes.com
Current Opinion in Biotechnology
|September 27, 2002
Abstract:
To date, the most widely used technology for conducting proteomic studies has been two-dimensional gel electrophoresis (2DGE), but this approach does have drawbacks. Isotope-coded affinity tagging (ICAT) is starting to challenge 2DGE as a new proteomic tool for the analysis of proteins in complex biological specimens. An appraisal of these two methodologies reveals that neither ICAT nor 2DGE provide comprehensive coverage on a proteome-wide scale.