Related Experiment Videos
Isolation of Friend leukemia virus resistant line from non-inbred mouse colony
Abstract:
Among a non-inbred ddY mouse colony, a small portion showed marked resistance to splenomegaly induction by Friend leukemia virus (FLV). The resistance to the virus appears to be governed by a single gene, and is dominant over the susceptibility. Thus, it was possible to breed two lines of mice, which are respectively resistant and susceptible to the virus. Resistant response to FLV of the resulting mice, which seemed to be homozygous for the dominant resistant gene, is similar to that of C57BL/6 and more rigid than that of BALB/c mice. Phenotype of F1, segregation ratio in F2 and BC1 hybrid progeny between resistant and susceptible lines were also compatible with the single gene model.
Insights
A single gene controls resistance to Friend leukemia virus (FLV) in ddY mice, with resistance being dominant. This genetic trait allows for the selective breeding of resistant and susceptible mouse lines for further study.
Area of Science:
- Virology
- Immunology
- Genetics
Background:
- A subset of non-inbred ddY mice exhibits resistance to Friend leukemia virus (FLV)-induced splenomegaly.
- This resistance phenotype suggests a genetic basis for differential host response to viral infection.
Purpose of the Study:
- To investigate the genetic control of resistance to Friend leukemia virus (FLV) in ddY mice.
- To establish and characterize genetically distinct mouse lines resistant and susceptible to FLV.
Main Methods:
- Selective breeding of ddY mice based on their response to FLV challenge.
- Analysis of F1, F2, and backcross (BC1) hybrid progeny to determine segregation patterns.
- Comparison of resistant mouse phenotype with known resistant strains (e.g., C57BL/6).
Main Results:
- Resistance to FLV-induced splenomegaly is governed by a single gene.
- The resistance allele is dominant over the susceptibility allele.
- Breeding experiments confirmed Mendelian inheritance patterns consistent with a single gene model.
Conclusions:
- A single, dominant gene controls FLV resistance in ddY mice.
- The established resistant mouse line provides a valuable model for studying viral pathogenesis and host immunity.
- Further research can utilize these distinct lines to elucidate the molecular mechanisms underlying FLV resistance.