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Updated: Sep 11, 2026

Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism
Published on: May 31, 2018
An experimental paradigm for studying the cellular and molecular mechanisms of cancer inhibition by energy
Zongjian Zhu1, Weiqin Jiang, Henry J Thompson
1Center for Nutrition in the Prevention of Disease, AMC Cancer Research Center, Denver, Colorado 80214, USA.
Abstract:
With a rapid-emergence, chemically induced animal model for breast cancer, an experiment designed to test the hypothesis that energy restriction (ER) induces the loss of carcinogen-initiated cells from the mammary gland, thereby conferring a permanent protective effect against the development of cancer, failed to support this hypothesis. Nonetheless, this experiment served to define an experimental approach and a time frame on which to focus mechanistic inquiry. With an ER and energy repletion (ER-REP) protocol as a tool for identifying potential mediators of the cancer-inhibitory activity of ER, concomitant changes in plasma corticosterone and insulin-like growth factor 1 during energy restriction and repletion were observed. The relationship of the timing of these hormonal changes to the time frame of change in the carcinogenic response during ER-REP was consistent with the role of both hormones in mediating the protective effects of ER. However, a similar pattern of change in the energy-regulated hormone leptin indicated that its role in cancer inhibition also merits consideration.
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