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Updated: Aug 8, 2026
10:53
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Published on: January 16, 2017
Possible physiological role of H19 RNA
Suhail Ayesh1, Imad Matouk, Tamar Schneider
1Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel.
Molecular Carcinogenesis
|September 27, 2002
Summary
The H19 gene
Area of Science:
- Molecular biology
- Cancer research
- Genetics
Background:
- The H19 gene's product is an untranslated RNA with an undetermined function.
- The H19 gene is implicated in oncofetal development.
Purpose of the Study:
- To investigate the function of H19 RNA.
- To identify genes modulated by H19 RNA.
- To explore the role of H19 RNA in cancer progression.
Main Methods:
- Human cDNA Atlas microarray analysis to detect gene expression changes.
- Cell culture experiments under varying serum conditions.
- Analysis of cell growth advantages and stress responses.
Main Results:
- H19 RNA upregulates genes associated with cell invasion, migration, and angiogenesis.
- H19 RNA confers a growth advantage in serum-poor conditions, but not in rich medium.
- H19-expressing cells show impaired induction of p57(kip2) under serum stress.
Conclusions:
- H19 RNA may promote cancer progression, angiogenesis, and metastasis.
- The H19 gene's role in cellular behavior is context-dependent, particularly concerning serum availability.
- Dysregulation of p57(kip2) induction might be a mechanism through which H19 influences cell survival.
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