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[Photometric method for determining ethanol].

Iu V Rodionov1, O I Keppen, M V Sukhacheva

  • 1Moscow State University, Department of Biology, Moscow, 119899 Russia.

Prikladnaia Biokhimiia I Mikrobiologiia
|September 28, 2002
PubMed
Summary

A new enzymatic assay accurately measures ethanol in biological samples like saliva and blood serum. This sensitive method is unaffected by other compounds and can also detect methanol and propanol.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry

Context:

  • Accurate ethanol determination is crucial in clinical diagnostics and forensic science.
  • Existing methods may lack sensitivity or specificity for biological fluid analysis.

Purpose:

  • To develop a novel, sensitive, and specific enzymatic photometric assay for ethanol determination.
  • To validate the assay's applicability in biological fluids and assess interference from other compounds.

Summary:

  • A new assay utilizes alcohol oxidase and peroxidase for sensitive ethanol quantification.
  • The method demonstrates high specificity, with no interference from secondary alcohols or other organic compounds.
  • Applicable to biological samples (saliva, blood serum) using standard spectrophotometers or photoelectric colorimeters; also capable of determining methanol and propanol.

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Impact:

  • Provides a reliable and accessible method for ethanol analysis in various biological and chemical contexts.
  • Facilitates accurate ethanol level monitoring in clinical and research settings.
  • Expands analytical capabilities for related alcohol compounds like methanol and propanol.