Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

[Photometric method for determining ethanol].

Iu V Rodionov¹, O I Keppen, M V Sukhacheva

¹Moscow State University, Department of Biology, Moscow, 119899 Russia.

Prikladnaia Biokhimiia I Mikrobiologiia

|September 28, 2002

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Proteomic and transcriptomic analyses of <i>Cutibacterium acnes</i> biofilms and planktonic cultures in presence of epinephrine.

AIMS microbiology·2024

Same author

Epinephrine affects gene expression levels and has a complex effect on biofilm formation in <i>M</i> <i>icrococcus luteus</i> strain C01 isolated from human skin.

Biofilm·2021

Same author

[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].

Voprosy pitaniia·2020

Same author

[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].

Voprosy pitaniia·2019

Same author

[Molecular and genetic studies of genetically engineered potato: transformation event PH05-026-0048].

Voprosy pitaniia·2018

Same author

[Typing of the Closely Related Strains of Euryarchaeal Genus Acidiplasma (Thermoplasmatales) Using REP-PCR DNA Fingerprinting Technique].

Mikrobiologiia·2016

A new enzymatic assay accurately measures ethanol in biological samples like saliva and blood serum. This sensitive method is unaffected by other compounds and can also detect methanol and propanol.

Area of Science:

Biochemistry
Analytical Chemistry

Context:

Accurate ethanol determination is crucial in clinical diagnostics and forensic science.
Existing methods may lack sensitivity or specificity for biological fluid analysis.

Purpose:

To develop a novel, sensitive, and specific enzymatic photometric assay for ethanol determination.
To validate the assay's applicability in biological fluids and assess interference from other compounds.

Summary:

A new assay utilizes alcohol oxidase and peroxidase for sensitive ethanol quantification.
The method demonstrates high specificity, with no interference from secondary alcohols or other organic compounds.
Applicable to biological samples (saliva, blood serum) using standard spectrophotometers or photoelectric colorimeters; also capable of determining methanol and propanol.

Related Experiment Videos

Impact:

Provides a reliable and accessible method for ethanol analysis in various biological and chemical contexts.
Facilitates accurate ethanol level monitoring in clinical and research settings.
Expands analytical capabilities for related alcohol compounds like methanol and propanol.