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[Photometric method for determining ethanol]
Iu V Rodionov1, O I Keppen, M V Sukhacheva
1Moscow State University, Department of Biology, Moscow, 119899 Russia.
Prikladnaia Biokhimiia I Mikrobiologiia
|September 28, 2002
Abstract:
A novel enzymatic photometric assay for ethanol determination using alcohol oxidase and peroxidase is described. The sensitivity of the method allows detecting ethanol in biological fluids (saliva and blood serum). Secondary alcohols and other organic compounds do not interfere with the assay. General-purpose spectrophotometers and photoelectric colorimeters can be used in the measurements. Methanol and propanol can also be determined by this technique.