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Updated: Jul 6, 2026
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
Iodoinsulin used to determine specific activity of iodine-131
Anodal mobility of iodoinsulin on starch-gel electrophoresis increases progressively as the number of iodine atoms substituted in the molecule increases. The iodine content of iodine-131 solutions is determined by comparison of autoradiographic patterns of insulin heavily labeled with iodine-131 and of insulins lightly labeled with iodine-131 and known quantities of iodine-127.
Anodal mobility of iodoinsulin on starch-gel electrophoresis increases progressively as the number of iodine atoms substituted in the molecule increases. The iodine content of iodine-131 solutions is determined by comparison of autoradiographic patterns of insulin heavily labeled with iodine-131 and of insulins lightly labeled with iodine-131 and known quantities of iodine-127.
