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Quantitation of gene expression by RT-PCR and HPLC analysis of PCR products

Franz Bachmair1, Christian G Huber, Guenter Daxenbichler

  • 1Department of Obstetrics and Gynecology, University Hospital, University of Innsbruck, Austria.

Methods in Molecular Biology (Clifton, N.J.)
|September 28, 2002
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...