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Related Experiment Videos

Ouabain-sensitive adenosine triphosphatase from human kidneys.

B R Nechay, J A Nelson, R R Contreras

    The Journal of Pharmacology and Experimental Therapeutics
    |February 1, 1975
    PubMed
    Summary

    Human kidney ATPase activity varies by region, with cortical enzyme levels decreasing during preservation. Ouabain-sensitive ATPase in kidney cortex and medulla share similar properties and sensitivities to inhibitors.

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    Area of Science:

    • Biochemistry
    • Nephrology
    • Transplantation Science

    Background:

    • Adenosine triphosphatase (ATPase) is crucial for kidney function.
    • Understanding ATPase activity is vital for assessing kidney viability during preservation for transplantation.

    Purpose of the Study:

    • To investigate the characteristics of ouabain-sensitive ATPase in human cadaver kidneys.
    • To evaluate how kidney preservation affects ATPase activity in different renal regions.

    Main Methods:

    • Studied ATPase activity in tissue homogenates and subcellular fractions of human cadaver kidneys.
    • Analyzed ouabain-sensitive ATPase activity in renal cortex, medulla, and papilla.
    • Assessed enzyme kinetics and inhibition by ouabain and other agents.

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    Main Results:

    • Ouabain-sensitive ATPase activity was highest in the medulla, intermediate in the cortex, and lowest in the papilla.
    • Cortical ATPase activity decreased over time during kidney preservation.
    • The enzyme from cortex and medulla showed similar requirements for ions and ATP, and similar ouabain sensitivity.
    • Potassium (K+) antagonized ouabain inhibition, and various heavy metal compounds inhibited the enzyme.

    Conclusions:

    • Regional differences in ATPase activity exist within the human kidney.
    • Kidney preservation impacts cortical ATPase activity, suggesting potential functional changes.
    • The ouabain-sensitive ATPase enzyme in human kidney cortex and medulla shares significant biochemical properties.