In vitro cultivation and identification of aortic endothelium from miniature pig

Pieces of miniature pig aorta were explanted endothelial face down directly on the bottom of Petri dishes. On removal of the aorta fragments after 2-4 days of incubation single cells or small groups of viable endothelium were found attached. The cells developed into a permanent line (at present over 18 passages) and were identified as pure endothelial cells both by light- and electron microscopic examination.