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Separation of salidroside from Rhodiola crenulata by high-speed counter-current chromatography
Xiao Han1, Tianyou Zhang, Yun Wei
1Beijing Research Center for Separation & Purification Technologies of Natural Products, Beijing Institute of New Technology Application, China.
Journal of Chromatography. A
|September 28, 2002
Abstract:
High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate-n-butanol-water (1:4:5, v/v) in the first run and chloroform-methanol-isopropanol-water (5:6:1:4) in the second run. The method yielded 21.9 mg of salidroside from 1.216 g of the crude sample at 98% purity determined by HPLC analyses. Identification was performed by 1H NMR, 13C NMR, and MS.