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Updated: Sep 29, 2026

Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
Published on: October 16, 2016
ASI1, a gene encoding a novel leucine zipper protein, is induced during development of the macronucleus in
Rupa A Udani1, Kathleen M Karrer
1Department of Biological Sciences, Marquette University, Milwaukee, WI 53201-1881, USA.
Abstract:
Sexual reproduction in the ciliate Tetrahymena follows a complex developmental program involving the sequential regulation of dozens of genes. Genes that are up-regulated during post-zygotic development in Tetrahymena were isolated by subtractive hybridization. Anlagen stage induced gene 1 (ASI1) encodes a 2.8 kb transcript that contains a single intron and is induced during macronuclear development. ASI1 is a single copy gene in both the micronucleus and the macronucleus. It encodes a 95 kDa conceptual protein with a leucine zipper near the amino terminus.
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