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Related Experiment Videos

Microarray analysis of gene expression changes in aging in mouse submandibular gland.

K Hiratsuka1, Y Kamino, T Nagata

  • 1Department of Biochemistry, Nihon Unversity School of Dentistry at Matsudo, Matsudo, Chiba, Japan.

Journal of Dental Research
|September 28, 2002
PubMed
Summary

Aging significantly alters gene expression in mouse salivary glands, with most changes involving decreased activity. DNA array technology aids in identifying age-related genes for future aging research.

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Area of Science:

  • Genomics
  • Aging Research
  • Salivary Gland Biology

Background:

  • Salivary gland function during aging is poorly understood at the gene expression level.
  • DNA array technology offers a sensitive method for global gene expression analysis.

Purpose of the Study:

  • To investigate age-associated changes in gene expression within the mouse submandibular gland (SMG).
  • To identify candidate genes affected by aging using high-density oligonucleotide arrays.

Main Methods:

  • Comparison of gene expression profiles in submandibular glands of adult versus elderly mice.
  • Utilized high-density oligonucleotide arrays for global gene expression screening.
  • Analyzed over 1300 genes for significant expression level changes.

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Main Results:

  • 160 out of 1328 screened genes (12.0%) showed more than a two-fold change in expression.
  • 154 genes (96.3%) exhibited decreased expression in elderly mice, including those involved in transcription, transport, and signaling.
  • Only 6 genes (3.7%) showed increased expression in elderly mice.

Conclusions:

  • Aging in mouse SMG is characterized by a predominant decrease in gene expression rather than an increase.
  • DNA array technology is a valuable tool for discovering age-associated genes in aging studies.