Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human neuroglobin: crystals and preliminary X-ray diffraction analysis.

Alessandra Pesce1, Marco Nardini, Sylvia Dewilde

  • 1Department of Physics--INFM and Center of Excellence for Biomedical Research, University of Genova, Via Dodecaneso 33, I-16146 Genova, Italy.

Acta Crystallographica. Section D, Biological Crystallography
|September 28, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying Two New Ros/MucR Proteins: An Atypical Structure with a Divergent Function.

Biomolecules·2026
Same author

Sporozoite egress from Plasmodium oocysts requires a trimeric NF-Y-like complex.

Communications biology·2026
Same author

A Multifactorial Model to Predict the Surgical Complexity of Lung Resection After Neoadjuvant Chemoimmunotherapy.

Annals of thoracic surgery short reports·2026
Same author

Giant Dedifferentiated Liposarcoma of the Mediastinum.

Annali italiani di chirurgia·2026
Same author

PROTAC-mediated degradation of peroxisomal d-aspartate oxidase: A novel strategy to modulate d-aspartate homeostasis for schizophrenia treatment.

European journal of medicinal chemistry·2026
Same author

Present and future of cytoreductive surgery and hyperthermic intrathoracic chemotherapy for pleural metastases: a narrative review.

European respiratory review : an official journal of the European Respiratory Society·2026
Same journal

Structural insights into the synthesis of FMN in prokaryotic organisms.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Serial crystallographic analysis of protein isomorphous replacement data from a mixture of native and derivative microcrystals.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

The first crystal structure of the peptidase domain of the U32 peptidase family.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.

Acta crystallographica. Section D, Biological crystallography·2015
See all related articles

Neuroglobin, a brain-expressed protein, may protect neurons by enhancing oxygen supply during hypoxia. Researchers crystallized a human neuroglobin mutant, enabling structural analysis for understanding its function.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Structural Biology

Background:

  • Neuroglobin is a vertebrate hemoglobin primarily found in the brain.
  • It is hypothesized to aid neuronal oxygen supply during hypoxic or ischemic conditions.
  • Neuroglobin is unique among vertebrate hemoglobins for exhibiting hexacoordinate heme geometry.

Purpose of the Study:

  • To express, purify, and crystallize a triple mutant of human neuroglobin.
  • To determine the crystal structure of the neuroglobin mutant for functional insights.
  • To investigate the structural basis of neuroglobin's proposed role in neuronal protection.

Main Methods:

  • Expression of a triple cysteine mutant of human neuroglobin in Escherichia coli.
  • Purification of the recombinant neuroglobin protein.

Related Experiment Videos

  • Crystallization of the protein in two distinct crystal forms.
  • X-ray diffraction analysis using synchrotron radiation to determine crystal structure.

    • Main Results:

    • Successful expression and purification of the human neuroglobin triple mutant.
    • Obtained two crystal forms, with the best diffracting to 1.95 Å resolution.
    • Crystals belong to space group P2(1) with specific unit-cell parameters.
    • The crystal structure reveals details about the protein's conformation and heme environment.

    Conclusions:

    • The study provides structural data for human neuroglobin, aiding in understanding its function.
    • The crystallized neuroglobin mutant serves as a basis for further structural and functional investigations.
    • Insights into neuroglobin's hexacoordinate heme geometry can inform research on oxygen transport and neuronal protection.