Granada Crystallisation Box: a new device for protein crystallisation by counter-diffusion techniques
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Updated: Sep 29, 2026

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
Published on: August 14, 2018
Juan Ma Garcia-Ruíz1, Luis Antonio Gonzalez-Ramirez, José Antonio Gavira
1Laboratorio de Estudios Cristalográficos, IACT Campus Fuentenueva, Facultad de Ciencias, 18002-Granada, Spain. jmgruiz@ugr.es
Abstract:
Granada Crystallisation Box (GCB) is a new crystallisation device designed to perform counter-diffusion experiments. Here we describe the device and its use for protein crystallisation purposes. GCB allows one to explore and exploit the coupling between crystallisation and diffusion. The role of viscous fluids, gels and/or microgravity can be enhanced by using capillary volumes, creating a perfect diffusive mass transport scenario. The use of capillaries also reduces the consumption of macromolecules and avoids the handling of crystals for X-ray diffraction data collection.
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