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Related Experiment Videos

Round window gentamicin absorption: an in vivo human model.

Zoran Becvarovski1, Dennis I Bojrab, Elias M Michaelides

  • 1Department of Otolaryngology, Michigan Ear Institute, Farmington Hills, Michigan, USA. zb@ent.com.au

The Laryngoscope
|September 28, 2002
PubMed
Summary

Intratympanic gentamicin rapidly crosses the round window membrane into the inner ear fluid. This novel human model effectively measures drug absorption through the round window for otological medications.

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Area of Science:

  • Otolaryngology
  • Pharmacokinetics
  • Inner ear drug delivery

Background:

  • Assessing drug penetration into the inner ear is crucial for treating inner ear disorders.
  • The round window membrane is a key barrier for otical drug delivery.
  • A reliable method to measure drug absorption in vivo is needed.

Purpose of the Study:

  • To introduce and validate a novel human labyrinthine sampling model.
  • To measure in vivo gentamicin absorption through the round window membrane.
  • To assess the permeability of the round window to otological medications.

Main Methods:

  • Prospective study utilizing a novel human labyrinthine sampling model.
  • Gentamicin administered transtympanically or via facial recess approach.

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  • Labyrinthine fluid, serum, and cerebrospinal fluid samples collected and analyzed.

    • Main Results:

    • Intratympanic gentamicin achieved labyrinthine fluid concentrations of 0-16 mg/L.
    • Systemic absorption detected in 4/11 patients (serum levels 0.3-0.4 mg/L).
    • No gentamicin detected in cerebrospinal fluid.

    Conclusions:

    • Intratympanic gentamicin rapidly diffuses through the round window membrane.
    • Significant inner ear drug concentrations are achievable.
    • The model is suitable for assessing round window permeability to medications like steroids and antibiotics.