Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tissue Transplantation01:24

Tissue Transplantation

Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The anti-interleukin-6 antibody ALD518-P18 mitigates murine kidney ischemia-reperfusion injury.

Scientific reports·2026
Same author

Remodeling of the Mouse Liver and Skeletal Muscle Metabolome in Response to Continuous Acute Exercise and Disruption of AMPK-Glycogen Interactions.

Metabolites·2026
Same author

Perspectives on the 2026 ISHLT Consensus Statement on Clinical Cardiac Xenotransplantation.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2026
Same author

The 2026 International Society for Heart and Lung Transplantation Consensus Statement on clinical cardiac xenotransplantation.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2026
Same author

Comparison of Classical P81 and SVI-P Cation-Exchange Papers in Radiometric Protein Kinase Assays.

The Biochemical journal·2025
Same author

Targeting the complement lectin pathway with a highly specific MASP-2 inhibitor protects against renal ischemia-reperfusion injury.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Jun 29, 2026

Murine Skin Transplantation
06:42

Murine Skin Transplantation

Published on: January 16, 2008

Gal mismatch alone causes skin graft rejection in mice.

Hilton Gock, Lisa Murray-Segal, Evelyn Salvaris

    Transplantation
    |September 28, 2002
    PubMed
    Summary

    We developed a novel model for studying Galactose-alpha1,3-galactose (Gal) mismatch in xenografts. This model demonstrates that anti-Gal antibodies can cause acute rejection, while T-cells mediate chronic rejection, crucial for xenotransplantation research.

    More Related Videos

    Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice
    06:48

    Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

    Published on: July 25, 2014

    Murine Full-thickness Skin Transplantation
    07:59

    Murine Full-thickness Skin Transplantation

    Published on: January 2, 2017

    Related Experiment Videos

    Last Updated: Jun 29, 2026

    Murine Skin Transplantation
    06:42

    Murine Skin Transplantation

    Published on: January 16, 2008

    Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice
    06:48

    Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

    Published on: July 25, 2014

    Murine Full-thickness Skin Transplantation
    07:59

    Murine Full-thickness Skin Transplantation

    Published on: January 2, 2017

    Area of Science:

    • Transplantation Immunology
    • Xenotransplantation Research
    • Immunogenetics

    Background:

    • Galactose-alpha1,3-galactose (Gal) is a key xenoantigen in pig-to-human xenografts.
    • Eliminating Gal may improve xenograft survival but its essentiality in pigs is debated.
    • Understanding Gal-mediated immunity is vital for overcoming xenograft rejection.

    Purpose of the Study:

    • To establish a model system for studying xenograft rejection solely based on Gal mismatch.
    • To investigate the roles of innate and adaptive immunity in Gal-mediated rejection.
    • To assess the impact of anti-Gal antibodies on xenograft survival.

    Main Methods:

    • Analysis of Gal skin graft survival on Gal-deleted (Gal KO) mice.
    • Utilizing T and B cell deficient (RAG-1 KO) mice to study innate immunity.
    • Employing sensitization with primary allografts and passive immunization with anti-Gal antibodies.

    Main Results:

    • Gal KO mice showed chronic rejection of Gal skin grafts (25-48% by 100 days).
    • T and B cell deficient recipients did not reject grafts, indicating T-cell involvement in chronic rejection.
    • Sensitization and passive anti-Gal antibody transfer accelerated and induced rejection.

    Conclusions:

    • A model for Gal-mismatch xenograft rejection has been established.
    • Non-complement-fixing anti-Gal antibodies can mediate acute rejection.
    • T-cell-mediated chronic rejection presents a significant barrier in Gal-mismatch xenotransplantation.