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DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis
Hiroshi Yamasaki1, Minoru Nakao, Yasuhito Sako
1Department of Parasitology. Animal Laboratory for Medical Research, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa, Japan. hiyamasak@asahikawa-med.ac.jp
Journal of Clinical Microbiology
|October 2, 2002
Abstract:
For DNA differential diagnosis of human Taenia cestodes, a base excision sequence scanning thymine-base method using the cytochrome c oxidase subunit I and cytochrome b genes as targets was used. The characteristic thymine-base peak profiles provide four distinct types, unique for T. saginata, T. asiatica, and two genotypes of T. solium. This approach provides a useful tool for the identification and diagnosis of human taeniid cestodes without DNA sequencing if nucleotide sequence databases are available.