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Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development.

A. M. Koltunow1, J. Truettner, K. H. Cox

  • 1Department of Biology, University of California, Los Angeles, California 90024-1606.

The Plant Cell
|December 1, 1990
PubMed
Summary
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This study reveals distinct gene expression programs during tobacco anther development, correlating with specific cell types like the tapetum. Manipulating these programs can lead to male sterility, highlighting their importance in plant reproduction.

Area of Science:

  • Plant Molecular Biology
  • Reproductive Biology
  • Genetics

Background:

  • Anther development involves complex temporal and spatial gene regulation.
  • Specific cell types within the anther, such as the tapetum, exhibit unique molecular profiles.

Purpose of the Study:

  • To investigate the regulation of mRNA sequences specific to tobacco anther development.
  • To identify and characterize genes involved in anther cell differentiation and function.
  • To understand the role of tapetal gene expression in male fertility.

Main Methods:

  • Analysis of temporal and spatial mRNA accumulation patterns in tobacco anthers.
  • Isolation and characterization of a tapetal-specific gene (TA29).
  • Reporter gene assays (chimeric GUS and diphtheria toxin A-chain genes) to study gene regulation.

Related Experiment Videos

  • Functional analysis using a cytotoxic gene to ablate tapetum cells.

    • Main Results:

    • Three distinct mRNA sets were identified with specific temporal and spatial accumulation patterns during anther development.
    • The TA29 gene is transcriptionally regulated, with a 122-base pair 5' region controlling tapetal-specific expression.
    • Tapetum ablation resulted in male-sterile plants without affecting surrounding sporophytic tissues.

    Conclusions:

    • Anther development is governed by multiple independent gene expression programs linked to specific cell types.
    • Tapetal gene expression plays a crucial role in male fertility in tobacco.
    • The TA29 gene serves as a valuable tool for studying tapetal development and function.