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A reconstituted in vitro clot model for evaluating laser thrombolysis
Abram D Janis1, Lisa A Buckley, Abby N Nyara
1Oregon Medical Laser Center, Portland, OR 97225, USA.
Journal of Thrombosis and Thrombolysis
|October 2, 2002
Summary
A new reconstituted clot model offers a reproducible target for laser thrombolysis. This model effectively evaluates laser parameters for clot removal, showing minimal impact on removal rates when average power is constant.
Area of Science:
- Biomedical Engineering
- Medical Physics
- Vascular Surgery
Background:
- Laser thrombolysis aims to selectively remove blood clots using laser energy.
- Developing reliable models is crucial for optimizing laser parameters in clot removal.
Purpose of the Study:
- To develop and evaluate a reconstituted clot model for assessing laser thrombolysis.
- To determine the effect of various laser parameters on thrombus removal rate.
Main Methods:
- A reconstituted clot model was created with controlled fibrinogen concentrations and hematocrits.
- Ex vivo swine clots and gelatin phantoms were used for comparison.
- A 577 nm pulsed dye laser was employed to measure mass removal rates and ablation efficiencies.
Main Results:
- The reconstituted clot model demonstrated reproducible ablation efficiencies.
- Ex vivo clots showed significant variability (ANOVA p < 0.05).
- Varying laser pulse energy and repetition rate at constant average power did not significantly alter mass removal rates.
Conclusions:
- The reconstituted clot model is a viable, reproducible, and biologically relevant target for laser thrombolysis research.
- Ex vivo clots lack inter-individual reproducibility, and gelatin phantoms lack clinical relevance.
- Laser parameter adjustments at constant average power have limited clinical impact on mass removal rates.