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Related Experiment Videos

Solubilization limit of lysozyme into DODMAC reverse micelles.

Youn-Ok Shin1, Juan H Vera

  • 1Department of Chemical Engineering, McGill University, 3610 University Street, Montreal, Quebec, Canada, H3A 2B2.

Biotechnology and Bioengineering
|October 2, 2002
PubMed
Summary

Dioctyldimethyl ammonium chloride (DODMAC) facilitates lysozyme extraction into organic phases via reverse micelles. Lysozyme extraction efficiency depends on pH and aqueous cations, with a notable solubilization limit observed.

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Area of Science:

  • Biochemistry
  • Separation Science
  • Biotechnology

Background:

  • Reverse micelles offer a promising method for protein extraction.
  • Dioctyldimethyl ammonium chloride (DODMAC) is a surfactant used in micellar systems.
  • Lysozyme is a model protein for studying extraction processes.

Purpose of the Study:

  • To investigate the solubilization behavior of lysozyme in DODMAC reverse micelles.
  • To determine factors affecting lysozyme extraction efficiency and limits.
  • To understand the role of aqueous cations and surfactant concentration on protein recovery.

Main Methods:

  • Utilized DODMAC to form reverse micelles for lysozyme extraction.
  • Varied parameters including pH, aqueous cation type, temperature, and DODMAC concentration.

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  • Analyzed lysozyme concentration in both aqueous and organic phases.

    • Main Results:

    • Complete lysozyme removal from aqueous phase achieved at pH > pI.
    • A solubilization limit for lysozyme in the organic phase was identified, forming a precipitate.
    • Extraction efficiency was highly sensitive to aqueous cation type, less so to temperature and DODMAC concentration.
    • Increased DODMAC concentration (100-200 mM) showed minimal impact on maximum lysozyme concentration.

    Conclusions:

    • Lysozyme extraction using DODMAC reverse micelles is feasible but limited by solubilization capacity.
    • Lysozyme-cation interactions significantly influence extraction efficiency.
    • Optimizing pH and understanding cation effects are crucial for effective lysozyme recovery.