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Related Experiment Videos

Quantitative proteomic analysis of Myc oncoprotein function.

Yuzuru Shiio1, Sam Donohoe, Eugene C Yi

  • 1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA.

The EMBO Journal
|October 3, 2002
PubMed
Summary
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The Myc oncoprotein promotes cell growth by altering protein expression, impacting cell mass and morphology. This study reveals Myc

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Proteomics

Background:

  • The Myc oncoprotein is a key regulator of cell proliferation and growth.
  • Understanding Myc's function requires detailed analysis of its impact on cellular protein expression.
  • Quantitative proteomics offers a powerful approach to study complex cellular processes.

Purpose of the Study:

  • To investigate the functional role of the Myc oncoprotein in mammalian cells using quantitative proteomics.
  • To identify global protein expression changes induced by Myc.
  • To elucidate Myc's effects on cell growth, morphology, and cytoskeletal organization.

Main Methods:

  • Application of isotope-coded affinity tag (ICAT) reagent labeling for quantitative proteomics.
  • Utilizing tandem mass spectrometry to analyze differential protein expression.

Related Experiment Videos

  • Comparison of protein expression profiles between Myc-null and Myc-plus rat cells.

    • Main Results:

    • Identification of a differential protein expression catalog regulated by Myc.
    • Observed reduction in proteases and upregulation of anabolic enzymes, suggesting increased cell mass.
    • Detected alterations in adhesion molecules, actin network proteins, and Rho pathway proteins, leading to changes in cell morphology and focal adhesions.

    Conclusions:

    • Myc plays a novel role in regulating the cell cytoskeleton.
    • Myc-mediated changes in protein expression contribute to increased cell growth and altered cell morphology.
    • Quantitative whole-proteome analysis is a feasible method for studying mammalian cell functions.