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An LC/DAD-UV/MS method for the rapid detection of aristolochic acid in plant preparations
Jean-Robert Ioset1, Guy Emmanuel Raoelison, Kurt Hostettmann
1Institut de Pharmacognosie et Phytochimie, BEP, Université de Lausanne, Lausanne, Switzerland.
Planta Medica
|October 3, 2002
Abstract:
A new method using direct on-line coupling between HPLC and UV-DAD/MS was developed for the rapid detection of aristolochic acid I in plant preparations. The use of both UV-DAD and MS detectors enables this method to be very selective. A ng range detection limit was determined for both UV and MS detections. A quantitative determination of aristolochic acid I in plant preparations was achieved using UV and MS detection. A thin layer chromatography assay was also developed for the preliminary detection of aristolochic acid I in complex mixtures.