Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thymosin beta4 regulation, expression and function in aortic valve interstitial cells.

Quan-Yi Li1, Peter L Jones, Robert P Lafferty

  • 1Division of Cardiology, Abramson Pediatric Research Center, The Children's Hospital of Philadelphia, PA 19104, USA.

The Journal of Heart Valve Disease
|October 3, 2002
PubMed
Summary

Tenascin-C up-regulates thymosin beta-4 in aortic valve cells, potentially initiating valvular calcification. This extracellular matrix protein is linked to mineralization and matrix metalloproteinase-2 (MMP-2) activity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pyriphenone modification of glutaraldehyde pretreated bovine pericardium mitigates advanced glycation end products, calcification, and platelet adhesion.

Acta biomaterialia·2026
Same author

Identification of compounds that repress DUX4 expression in facioscapulohumeral muscular dystrophy.

Scientific reports·2026
Same author

Inhibition of the serotonin transporter and risk of heart valve disease: A systematic review and meta-analysis.

European journal of pharmacology·2026
Same author

Transgenic mouse models for investigating human DUX4 expression during development and its roles in FSHD pathophysiology.

Disease models & mechanisms·2026
Same author

Identification of compounds that repress DUX4 expression in facioscapulohumeral muscular dystrophy.

bioRxiv : the preprint server for biology·2026
Same author

Serotonin transporter downregulation is associated with aortic stenosis, and early profibrotic remodeling is mitigated by pharmacological inhibition of HTR2B receptor.

Frontiers in cardiovascular medicine·2026

Area of Science:

  • Cardiovascular Biology
  • Extracellular Matrix Research
  • Cellular Signaling

Background:

  • Tenascin-C, an extracellular matrix protein, is present in calcified aortic valves and associated with matrix metalloproteinase (MMP)-2.
  • Tenascin-C is absent in non-calcified human aortic valves.

Purpose of the Study:

  • To identify downstream targets of tenascin-C in aortic valve interstitial cells.

Main Methods:

  • Subtractive hybridization was employed using sheep aortic valve interstitial cells (SAVIC) cultured on collagen with or without tenascin-C.
  • Cell culture studies assessed thymosin beta-4 production and its effects on SAVIC.

Main Results:

  • Subtractive hybridization identified thymosin beta-4, an actin-binding protein, in nearly 70% of isolated clones.

Related Experiment Videos

  • Tenascin-C up-regulated thymosin beta-4 expression in SAVIC.
  • Thymosin beta-4 induced F-actin re-orientation and MMP-2 in SAVIC, though antisense oligonucleotides did not suppress MMP-2.

    • Conclusions:

    • Thymosin beta-4 is up-regulated by tenascin-C in aortic valve interstitial cells.
    • Thymosin beta-4 may play a role in the initial stages of valvular calcification.