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Updated: Jul 1, 2026

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
Published on: August 20, 2012
A FRET-based ultraviolet to near-infrared frequency converter
Jason M Serin1, Darryl W Brousmiche, Jean M J Fréchet
1Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA.
Abstract:
The synthesis and photophysical characterization of a generation 1 dendrimer containing four coumarin 2 laser dyes at the periphery and a perylenebis(dicarboximide) derivative at the core are described. It was found that 99% of the UV light absorbed by the peripheral coumarin 2 donor chromophores is transferred through fluorescence resonance energy transfer (FRET) to the core acceptor. Emission from the core via FRET is observed in the near-infrared with a 6.2-fold amplification relative to direct excitation.
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