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Rapid detection of Obesumbacterium proteus from yeast and wort using polymerase chain reaction
1Brewing Research International, Lyttel Hall, Nutfield, Surrey R.H.1. 4HY, UK.
Aims:
To design and evaluate PCR primers for the rapid detection of Obesumbacterium proteus.
Methods And Results:
The 16S rDNA from a wild-type Obesumbacterium proteus biotype II isolate was sequenced and the resultant data used to produce specific primers for this organism. These primers discriminated between biotype I (nonbrewery) and biotype II isolates. In addition, the primers were able to detect Obesumbacterium proteus in wort and in yeast slurries in the presence of competitive bacteria. The primers were tested against a range of other beer spoilage bacteria for any cross-reactions. None were detected.
Conclusions:
Obesumbacterium proteus primers can detect this contaminant without generating cross-reactions to related species.
Significance And Impact Of The Study:
The primers generated in this study can now be used for PCR detection assays that will contribute to early detection of this important process contaminant.