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Pru p 3 (LTP) content in peach extracts.
J Carnés1, E Fernández-Caldas, M T Gallego
1C.B.F. LETI, S.A. Research Laboratories, Calle del Sol 5, 28760 Tres Cantos, Madrid, Spain.
Allergy
|October 3, 2002
Summary
Peach peel contains significantly higher levels of lipid transfer proteins (LTPs) than pulp. This study purified LTP and developed a method to quantify it, confirming LTP
Area of Science:
- Food science and allergy research.
- Biochemistry of plant proteins.
Background:
- Lipid transfer proteins (LTPs) are common in fruits.
- LTP sensitization frequently occurs in patients with fruit allergies.
Purpose of the Study:
- To purify LTP from peach.
- To quantify LTP content in peach peel and pulp extracts.
- To evaluate LTP's role in peach allergy.
Main Methods:
- LTP purification using column chromatography.
- Production of polyclonal antibodies against LTP.
- Quantification of LTP via ELISA inhibition.
Main Results:
- Purified LTP identified at approximately 9 kDa.
- LTP concentration in peach peel is ~7x higher than in pulp.
- 83.3% of peach-allergic patients showed positive skin test to purified LTP.
Conclusions:
- A reliable method for quantifying LTP in peach extracts was established.
- Peach peel is a richer source of LTP compared to pulp.
- Findings support LTP's significance in peach allergy.