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Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
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Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
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The spindle checkpoint: structural insights into dynamic signalling.

Andrea Musacchio1, Kevin G Hardwick

  • 1Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy. amusacch@ieo.it

Nature Reviews. Molecular Cell Biology
|October 3, 2002
PubMed
Summary
This summary is machine-generated.

The spindle checkpoint ensures accurate chromosome segregation by monitoring microtubule-kinetochore interactions. This review integrates protein dynamics and structural data to clarify its molecular mechanisms.

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Related Experiment Videos

Last Updated: May 14, 2026

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

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Published on: September 17, 2012

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Chromosome segregation is vital for cell division fidelity.
  • The spindle checkpoint is crucial for preventing aneuploidy.
  • Understanding its molecular mechanisms is an ongoing challenge.

Purpose of the Study:

  • To review and integrate current findings on spindle checkpoint protein dynamics.
  • To connect kinetochore protein behavior with signaling complex structures.
  • To enhance understanding of mitotic fidelity mechanisms.

Main Methods:

  • Literature review and data integration.
  • Analysis of checkpoint protein dynamics at kinetochores.
  • Examination of structural information on signaling complexes.

Main Results:

  • Checkpoint proteins exhibit dynamic behavior at kinetochores.
  • Specific structural features of signaling complexes are elucidated.
  • The interplay between protein dynamics and structure is highlighted.

Conclusions:

  • The spindle checkpoint ensures chromosome segregation accuracy through dynamic protein interactions.
  • Integrating kinetic and structural data provides molecular insights.
  • Further research can refine our understanding of error correction during mitosis.