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Updated: Aug 23, 2026

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Antifungal effects of endodontic medicaments
J F Siqueira1, I N Rôças, F A Magalhães
1Department of Endodontics, Estácio de Sá University, Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. siqueira@estacio.br
Abstract:
The eradication of root canal infection is paramount in endodontic treatment. Because fungi are involved in some types of root canal infections, the purpose of this study was to investigate the antifungal effects of several medicaments against the following selected fungal species: Candida albicans, Candida glabrata, Candida guilliermondii, Candida parapsilosis, and Saccharomyces cerevisiae. The agar diffusion test was the method used. Calcium sulphate or zinc oxide in glycerin showed no inhibitory effects towards any selected fungal species. The pastes of calcium sulphate or calcium hydroxide in camphorated paramonochlorophenol (CPMC)/glycerin showed the most pronounced antifungal effects. Calcium hydroxide in glycerin or chlorhexidine, and chlorhexidine in a detergent also showed antifungal activity, which was, however, much lower than the pastes of calcium sulphate or calcium hydroxide in CPMC/glycerin. Strategies using medicaments that have antifungal effects may assist in the successful management of persistent or secondary endodontic infections caused by fungi.
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