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Rab11-FIP2, an adaptor protein connecting cellular components involved in internalization and recycling of epidermal

Donald N Cullis1, Betsey Philip, James D Baleja

  • 1Department of Biochemistry, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.

The Journal of Biological Chemistry
|October 5, 2002
PubMed

Insights

Rab11-FIP2 protein binds Reps1 and alpha-adaptin, suggesting a role in receptor-mediated endocytosis. Overexpression of Rab11-FIP2 specifically inhibits epidermal growth factor receptor internalization.

Area of Science:

  • Cell biology
  • Molecular biology
  • Endocytosis research

Background:

  • Rab11-binding proteins are involved in recycling endosome function.
  • Receptor-mediated endocytosis is a key cellular process for internalizing molecules.
  • EH domain proteins and AP-2 complexes play roles in endocytosis.

Purpose of the Study:

  • To investigate the potential role of Rab11-FIP2 in receptor-mediated endocytosis.
  • To identify binding partners of Rab11-FIP2 involved in endocytic pathways.
  • To determine the effect of Rab11-FIP2 on the internalization of specific receptors.

Main Methods:

  • Analysis of Rab11-FIP2's NPF motif for Reps1 binding.
  • Assessment of Rab11-FIP2 association with the alpha-adaptin subunit of AP-2 complexes.
  • Overexpression studies to observe the impact on epidermal growth factor receptor and transferrin receptor internalization.

Main Results:

  • Rab11-FIP2 possesses an NPF motif enabling binding to Reps1, an EH domain protein.
  • Rab11-FIP2 interacts with alpha-adaptin, a component of AP-2 complexes crucial for receptor recruitment.
  • Overexpression of Rab11-FIP2 selectively inhibits epidermal growth factor receptor internalization, not transferrin receptor uptake.

Conclusions:

  • Rab11-FIP2 may link receptor-mediated endocytosis to endosomal sorting via interactions with Rab11, Reps1, and alpha-adaptin.
  • The findings suggest Rab11-FIP2's specific involvement in the endocytosis pathway of certain receptors, like the epidermal growth factor receptor.
  • Rab11-FIP2's function extends beyond recycling endosomes to potentially regulate initial endocytic events.

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