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In vivo modulation of androgen receptor by androgens
V L Kumar1, P K Majumder, V Kumar
1Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110 029, India. kumarvl98@hotmail.com
Aim:
To study the effect of androgen and antiandrogen on the level of androgen receptor (AR) mRNA.
Methods:
The total RNA was extracted from the prostate and analyzed by slot blot analysis. The blots were hybridized with AR cDNA probe and 1A probe (internal control) and autoradiography was performed. The intensity of signal was measured with a densitometer and the ratio of AR RNA and 1A RNA was calculated.
Results:
Androgenic deprivation produced by castration decreased the weight of the prostate and increased the levels of AR mRNA. Treatment of the castrated rats with testostrone increased the weight of prostate and decreased the levels of AR mRNA. Treatment of normal rats with flutamide decreased the weight of the gland and increased the levels of AR mRNA.
Conclusion:
Androgens produce proliferative effect on the prostate and negatively regulate the AR transcription.