Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methods in virus diagnosis: immunofluorescence revisited.

C R Madeley1, J S M Peiris

  • 1Professor Emeritus of Clinical Virology, University of Newcastle upon Tyne, Newcastle upon Tyne, UK. dickmadeley@aol.com

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|October 9, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single amino acid substitution and inter-species transmission of MERS-coronavirus from camels to humans: abridged secondary publication.

Hong Kong medical journal = Xianggang yi xue za zhi·2024
Same author

Surveillance of environmental contamination by antibiotics and antibiotic-resistant pathogens: abridged secondary publication.

Hong Kong medical journal = Xianggang yi xue za zhi·2023
Same author

Maternal antibody against influenza neuraminidase in newborns: abridged secondary publication.

Hong Kong medical journal = Xianggang yi xue za zhi·2023
Same author

Viral burden and diversity in acute respiratory tract infections in hospitalized children in wet and dry zones of Sri Lanka.

PloS one·2021
Same author

Tropism of the novel human betacoronavirus lineage C virus in human ex vivo and in vitro cultures: potential transmissibility and pathogenesis in humans (abridged secondary publication).

Hong Kong medical journal = Xianggang yi xue za zhi·2021
Same author

Effect of increased influenza and pneumococcal vaccine coverage on the burden of influenza among elderly people in Hong Kong versus Brisbane: abridged secondary publication.

Hong Kong medical journal = Xianggang yi xue za zhi·2020

Immunofluorescence (IF) remains a valuable tool for rapid and accurate virus diagnosis. Its flexibility and specimen quality feedback enhance clinical dialogue and diagnostic services.

Area of Science:

  • Medical diagnostics
  • Virology
  • Laboratory medicine

Background:

  • Immunofluorescence (IF) is a widely used laboratory technique for virus diagnosis.
  • IF is facing declining use due to budget constraints and increasing demands for objective quality control.

Purpose of the Study:

  • To review the current status, value, and benefits of immunofluorescence in diagnostics.
  • To advocate for the continued use of IF in routine virus diagnosis.

Main Methods:

  • Literature review on the application and benefits of immunofluorescence.
  • Analysis of IF's advantages compared to other diagnostic methods.

Main Results:

  • IF is rapid, accurate with validated reagents, and flexible.

Related Experiment Videos

  • IF provides valuable feedback on specimen quality, fostering communication between labs and clinicians.
  • Rapid IF diagnosis for respiratory viruses significantly increases service utilization and epidemiological data.

    • Conclusions:

    • Immunofluorescence retains a crucial role in routine virus diagnosis.
    • The benefits of IF, including speed, accuracy, and specimen quality feedback, are not easily replicated by other techniques.
    • Continued use of IF enhances patient care, laboratory efficiency, and epidemiological surveillance.