Sequential analysis of nonstructural protein NSP4s derived from Group A avian rotaviruses

We determined the NSP4 sequences of turkey rotavirus strains Ty-1 and Ty-3 and a chicken rotavirus, strain Ch-1, and compared these sequences with those of a pigeon rotavirus, strain PO-13, and mammalian rotaviruses. The turkey strains and PO-13 were found to be closely related (90-97% homologies). Ch-1 NSP4 was distinctly different from other avian rotavirus NSP4s, with 78-79% homologies. The NSP4 sequences of avian rotaviruses were found to be 6-7 amino acids shorter than those of all mammalian strains and to have considerably low identities (31-37%) with them. Therefore, it seems highly likely that the NSP4 genes of avian rotaviruses are classified into two NSP4 genotypes distinct from those of mammalian rotaviruses. The enterotoxin domain in NSP4 is conserved in terms of its sequential and structural properties despite extremely low homologies in the full lengths of NSP4s in avian and mammalian rotaviruses.