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A polymorphic region in Mycobacterium abscessus contains a novel insertion sequence element.

Susan T Howard1, Thomas F Byrd2,1, C Richard Lyons3,1

  • 1University of New Mexico School of Medicine, Dept of Internal Medicine, 915 Camino de Salud, Albuquerque, NM 87131, USA1.

Microbiology (Reading, England)
|October 9, 2002
PubMed
Summary

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A novel insertion sequence, ISMab1, was discovered in Mycobacterium abscessus, an opportunistic pathogen. This finding reveals genetic diversity and potential horizontal gene transfer in this species.

Area of Science:

  • Microbiology
  • Genomics
  • Molecular Biology

Background:

  • Mycobacterium abscessus is an opportunistic pathogen with limited genetic characterization.
  • Insertion sequences (IS) are mobile genetic elements that can contribute to genomic plasticity.
  • Understanding genetic variation in M. abscessus is crucial for clinical and epidemiological studies.

Purpose of the Study:

  • To investigate a polymorphic region identified in Mycobacterium abscessus.
  • To characterize a novel insertion sequence, ISMab1, and its distribution in clinical isolates.
  • To explore the evolutionary relationships and potential horizontal gene transfer of ISMab1.

Main Methods:

  • Genomic subtraction was employed to identify a polymorphic region between a parental strain and a phenotypic variant.

Related Experiment Videos

  • The novel insertion sequence ISMab1 was identified and characterized within this polymorphic region.
  • Comparative sequence analysis and probing of clinical isolates were performed to assess ISMab1 distribution and genomic deletions.

    • Main Results:

    • A novel 1.7 kb insertion sequence, ISMab1, was discovered in a polymorphic region of M. abscessus.
    • ISMab1 exhibited high nucleotide identity to IS elements from other mycobacterial species, suggesting horizontal transfer.
    • ISMab1 was present as a single copy or absent in clinical isolates, and significant genomic deletions (>20 kb) were observed in some isolates.

    Conclusions:

    • The discovery of ISMab1 highlights genetic variability within Mycobacterium abscessus.
    • Evidence supports horizontal gene transfer as a mechanism for IS element dissemination among mycobacteria.
    • Genomic deletions and the presence/absence of ISMab1 contribute to the genetic diversity of M. abscessus clinical isolates.