Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Maize opaque endosperm mutations create extensive changes in patterns of gene expression.

Brenda G Hunter1, Mary K Beatty, George W Singletary

  • 1Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA.

The Plant Cell
|October 9, 2002
PubMed
Summary

Maize starchy endosperm mutants exhibit altered kernel properties. Gene expression analysis revealed distinct molecular groupings among these mutants, highlighting stress responses in opaque types.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression of progranulin (GP88) protein appears as an independent prognostic factor for clinical progression in high-risk prostate cancer patients.

Scientific reports·2026
Same author

A comparative approach for selecting orthologous candidate genes in genome-wide association studies across multiple species.

Plant physiology·2026
Same author

Prebiotic intervention changes host and microbe proteomes in plasma extracellular vesicles of Parkinson's disease.

Scientific reports·2026
Same author

Regular black tea kombucha consumption is associated with improved insulin resistance and increased ADIPOQ expression in adults with obesity: a pre-post clinical trial.

Food & function·2026
Same author

New Insights into Synergistic Boosts in SCFA Production Across Health Conditions Induced by a Fiber Mixture.

Nutrients·2025
Same author

A maize mutant in the glutamate receptor-like dwarf13 is modified by cis-acting natural variation and a cornichon homolog.

PLoS genetics·2025

Area of Science:

  • Plant Biology
  • Genetics
  • Biochemistry

Background:

  • Maize starchy endosperm mutants display undesirable kernel phenotypes, impacting texture and product quality.
  • The molecular basis for most of these mutants remains unknown, hindering a full understanding of their characteristics.

Purpose of the Study:

  • To investigate the biochemical and molecular underpinnings of various maize starchy endosperm mutants.
  • To correlate protein and amino acid profiles with mRNA transcript patterns to understand genotype-phenotype relationships.

Main Methods:

  • Characterization of protein and amino acid composition in nearly isogenic inbred lines of W64A maize mutants (o1, o2, o5, o9, o11, Mc, DeB30, fl2).
  • Analysis of endosperm mRNA transcript profiles using an Affymetrix GeneChip with over 1400 maize gene sequences at 18 days after pollination.

Related Experiment Videos

Main Results:

  • Significant reductions in zein protein synthesis were observed in W64A o2, DeB30, and fl2 mutants (35-55% of wild-type).
  • W64A o5, o9, o11, and Mc mutants showed zein levels at 80-90% of wild-type, with qualitative changes in o5 and Mc.
  • Opaque mutants exhibited pleiotropic gene expression alterations, including increased expression of stress and unfolded protein response genes.
  • Mutants were categorized into four groups based on global gene expression patterns.

Conclusions:

  • The study provides a molecular basis for understanding the diverse phenotypes of maize starchy endosperm mutants.
  • Gene expression profiling reveals common stress-related responses in opaque mutants, suggesting shared pathways.
  • Categorization based on gene expression offers a framework for future research into maize endosperm development and quality.