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Related Experiment Videos

Imaging gene expression and endogenous molecular processes: molecular imaging.

Ronald Blasberg1

  • 1Department of Neurology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA. blasberg@neuro1.mskcc.org

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
|October 9, 2002
PubMed
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Noninvasive molecular imaging uses reporter genes and probes to track biological processes without tissue sampling. This technology offers spatial and temporal insights into diseases, complementing traditional methods.

Area of Science:

  • Biomedical imaging
  • Molecular biology
  • Gene expression analysis

Background:

  • Noninvasive molecular imaging has advanced significantly, utilizing nuclear (PET, gamma camera), MRI, and optical systems.
  • Current strategies often employ indirect methods, coupling reporter genes with complementary probes to infer gene expression levels.

Purpose of the Study:

  • To review the development and applications of noninvasive molecular imaging techniques.
  • To highlight the value of reporter gene systems for monitoring molecular-genetic and cellular processes.
  • To discuss the translation of these technologies into clinical practice.

Main Methods:

  • Utilizing reporter gene constructs driven by specific promoters/enhancers.
  • Employing complementary reporter probes for detection.

Related Experiment Videos

  • Applying noninvasive imaging modalities like Positron Emission Tomography (PET).

    • Main Results:

    • Reporter gene expression can be continuous for tracking transduction sites and gene activity duration.
    • Reporter genes can be engineered for controlled expression or to sense endogenous promoter activity.
    • Successful imaging of endogenous processes like p53-dependent gene expression and T-lymphocyte activation in animal models.

    Conclusions:

    • Noninvasive molecular imaging provides a valuable spatial and temporal dimension to disease understanding.
    • These techniques complement traditional tissue-sampling assays.
    • Further development is needed for clinical translation of molecular imaging technologies.