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Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
Published on: January 18, 2014
Glutamate dehydrogenase activity: a major criterion for the selection of flavour-producing lactic acid bacteria
Catherine Tanous1, Agnieszka Kieronczyk, Sandra Helinck
1INRA, Unité de Biochimie et Structure des Protéines, 78352 Jouy-en-Josas.
Abstract:
Lactic acid bacteria (LAB) have the enzyme potential to transform amino acids into aroma compounds that contribute greatly to cheese flavour. Generally, amino acid conversion by LAB is limited by their low production of alpha-ketoglutarate since this alpha-ketoacid is essential for the first step of the conversion. Indeed, we have demonstrated that adding exogenous alpha-ketoglutarate to cheese curd, as well as using a genetically modified L. lactis strain capable of producing alpha-ketoglutarate from glutamate, greatly increased the conversion of amino acid to potent aroma compounds in cheese. Here we report the presence of glutamate dehydrogenase (GDH) activity required for the conversion of glutamate to alpha-ketoglutarate in several 'natural' LAB strains, commonly used in cheese manufacturing. Moreover, we show that the ability of LAB to produce aroma compounds from amino acids is closely related to their GDH activity. Therefore, GDH activity appears to be a major criterion for the selection of flavour-producing LAB strains, which could be used as a starter or as an adjunct to intensify flavour formation in some cheeses.
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