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A WD-repeat-containing putative regulatory protein in anthocyanin biosynthesis in Perilla frutescens
Kanokporn Sompornpailin1, Yukiko Makita, Mami Yamazaki
1Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University, Japan.
Abstract:
The WD-repeat proteins are found in eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as signal transduction, transcription, and proliferation. In this study, we have isolated a cDNA encoding a novel WD-repeat protein, PFWD, from the anthocyanin-pigmented leaves of Perilla frutescens using AN11 cDNA from Petunia hybrida as the probe. The C-terminal region of PFWD contains a WD repeat that is highly conserved in homologous proteins from a variety of organisms that do not produce anthocyanin such as yeast, nematodes and mammals. Transgenic Arabidopsis plants overexpressing PFWD exhibited phenotypic changes including enhancement of anthocyanin production and reduced viability. A study of the interaction between PFWD and anthocyanin regulatory proteins using a yeast two-hybrid system showed strong interaction between PFWD and MYC-RP, a MYC-like protein from P. frutescens. PFWD fusion proteins transiently expressed in onion epidermal cells were localized in the cytosol under both dark and light conditions. However, co-expression of PFWD and MYC-RP fusion proteins resulted in nuclear localization of PFWD. We propose a model of genetic regulation in which the PFWD protein acts in signal transduction process in a variety of pathways through protein interaction with MYC proteins.
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