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Related Experiment Videos

Automated protein design and sequence optimisation: scoring functions and the search problem.

A P Cootes1, P M Curmi, A E Torda

  • 1Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia. cootes@rsc.anu.edu.au

Current Protein & Peptide Science
|October 9, 2002
PubMed
Summary

Predicting the ideal protein sequence for a specific fold remains challenging due to difficulties in scoring sequence-structure pairs and the vast search space. Theoretical models struggle to keep pace with experimental successes in protein synthesis.

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Area of Science:

  • Molecular Biology
  • Biophysics
  • Computational Biology

Background:

  • Modern molecular biology enables the synthesis of diverse protein sequences.
  • However, theoretical approaches lag behind, struggling to predict optimal sequences for given protein structures.

Purpose of the Study:

  • To survey the key challenges in predicting ideal protein sequences for specific folds.
  • To discuss the limitations of current theoretical models in light of experimental advancements.

Main Methods:

  • Identification and discussion of critical aspects of sequence-structure prediction.
  • Survey of scoring methodologies for sequence-structure pairs.
  • Analysis of the vast sequence space and associated optimization problems.

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Main Results:

  • Lack of consensus on the best scoring methods for sequence-structure compatibility.
  • Uncertainty regarding whether score functions should represent free energy or abstract compatibility.
  • The astronomical number of possible sequences presents a significant optimization challenge.

Conclusions:

  • Predicting ideal protein sequences for a given fold is currently not reliably achievable.
  • Theoretical frameworks require further development to address scoring and search space complexities.
  • Recent experimental successes highlight the need for improved theoretical predictive power.