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The use of DODT as a non-malodorous scavenger in Fmoc-based peptide synthesis
A Teixeira1, W E Benckhuijsen, P E de Koning
1Department of Immunohematology and Bloodtransfusion, Building 1, E3-Q, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
Protein and Peptide Letters
|October 9, 2002
Abstract:
We have synthesized a random group of peptides and performed cleavages using various cleavage cocktails including 3,6-dioxa-1,8-octanedithiol (DODT). Purity of the peptides was compared to that obtained with standard protocols for cleavage using RP-HPLC and Maldi-Tof mass spectrometry. We show that stinking thiols can be replaced by the almost odourless (DODT) without negatively affecting the purity of the end product.