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Hyperbaric oxygen enhances apoptosis in hematopoietic cells
B J Ganguly1, N Tonomura, R M Benson
1Department of Veterinary and Animal Sciences and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Apoptosis : an International Journal on Programmed Cell Death
|October 9, 2002
Summary
Hyperbaric oxygen (HBO) therapy significantly increases hematopoietic cell apoptosis, both spontaneous and induced. This effect is linked to elevated intracellular hydrogen peroxide (H2O2) levels.
Area of Science:
- Cell Biology
- Biochemistry
- Physiology
Background:
- Hyperbaric oxygen (HBO) therapy involves administering 100% oxygen at elevated atmospheric pressure.
- Apoptosis, or programmed cell death, is a critical biological process.
- Understanding HBO's impact on cell death pathways is crucial for therapeutic applications.
Purpose of the Study:
- To investigate the effects of HBO on hematopoietic cell apoptosis.
- To determine if HBO enhances spontaneous and stimulus-induced apoptosis.
- To elucidate the underlying mechanisms, particularly the role of reactive oxygen species.
Main Methods:
- Exposure of HL-60 and Jurkat cells to HBO conditions (2.4 ATA, 97.9% O2).
- Assessment of apoptosis using various stimuli (gamma radiation, chemotherapy drugs, anti-Fas antibody).
- Measurement of intracellular hydrogen peroxide (H2O2) levels and the effect of dehydroascorbic acid.
Main Results:
- HBO significantly increased spontaneous apoptosis in HL-60 cells (42% at 12h) and Jurkat cells (44%).
- HBO enhanced apoptosis induced by gamma radiation (33%), melphalan (22%), camptothecin (13%), and chlorambucil (8%).
- HBO exposure increased intracellular H2O2 concentrations, and dehydroascorbic acid partially reversed these effects.
Conclusions:
- HBO therapy potently enhances both spontaneous and stimulus-induced apoptosis in various hematopoietic cell types.
- The pro-apoptotic effect of HBO is, at least partly, mediated by an increase in intracellular H2O2.
- HBO's impact on apoptosis does not appear to require new protein synthesis.
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