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Updated: Sep 29, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
A general synthesis of specifically deuterated nucleotides for studies of DNA and RNA
Bingzi Chen1, Elizabeth R Jamieson, Thomas D Tullius
1Department of Chemistry, Boston University, Boston, MA 02215, USA.
Abstract:
An efficient procedure is described for the preparation of ribonucleotides and deoxyribonucleotides with deuterium incorporated at the 1', 4', or 5' position. Three intermediates-[1-2H]-D-ribose, [4-2H]-D-ribose, and [5-2H(2)]-D-ribose-were prepared by chemical synthesis and subsequently converted to ribonucleotides and deoxyribonucleotides via enzymatic reactions. Milligram quantities of the desired products were obtained with an average deuterium content of 96+/-1%.
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