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Candihexin polyene macrolide complex: physicochemical characterization and antifungal activities of the single
Antimicrobial Agents and Chemotherapy
|August 1, 1975
Summary
Researchers isolated and purified four major candihexins (A, B, E, F) from Streptomyces viridoflavus. Candihexins A and B possess antifungal activity, while E and F do not, highlighting the importance of specific structural components.
Area of Science:
- Microbiology
- Natural Products Chemistry
- Medicinal Chemistry
Background:
- Streptomyces viridoflavus produces polyene macrolide antifungals.
- A natural mutant yields the candihexin complex, distinct from candidin.
Purpose of the Study:
- To resolve and characterize the candihexin complex.
- To determine the structural basis for antifungal activity.
Main Methods:
- Thin-layer chromatography for component separation.
- Isolation and purification of major candihexins (A, B, E, F).
- Hydrolysis and compositional analysis.
Main Results:
- The candihexin complex consists of eight components, with six possessing hexaene chromophores.
- Candihexins A and B are similar to candidin, containing mycosamine and exhibiting antifungal activity.
- Candihexins E and F have lower molecular weights, lack mycosamine, and show no antifungal activity.
Conclusions:
- Candihexins A and B are active antifungal macrolides.
- The presence of mycosamine is crucial for the antifungal activity of these compounds.
- Structural variations in candihexins impact their biological function.