Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression analysis in microdissected renal tissue. Current challenges and strategies.

Clemens D Cohen1, Matthias Kretzler

  • 1Medizinische Poliklinik, Ludwig-Maximilians-University of Munich, Germany.

Nephron
|October 10, 2002
PubMed
Summary

New gene expression techniques allow detailed study of kidney nephron segments. These methods aid in understanding kidney function differences and diagnosing renal diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Loss of genome maintenance is linked to mTOR complex 1 signaling and accelerates podocyte damage.

JCI insight·2025
Same author

Blood immunophenotyping identifies distinct kidney histopathology and outcomes in patients with lupus nephritis.

bioRxiv : the preprint server for biology·2024
Same author

Unbiased kidney-centric molecular categorization of chronic kidney disease as a step towards precision medicine.

Kidney international·2024
Same author

WNT-dependent interaction between inflammatory fibroblasts and FOLR2+ macrophages promotes fibrosis in chronic kidney disease.

Nature communications·2024
Same author

Rationale and design of the Nephrotic Syndrome Study Network (NEPTUNE) Match in glomerular diseases: designing the right trial for the right patient, today.

Kidney international·2024
Same author

Multi-omics data integration shines a light on the renal medulla.

Kidney international·2024

Area of Science:

  • Nephrology
  • Molecular Biology
  • Genomics

Background:

  • Kidney compartmentalization necessitates microtechniques for studying nephron segment functional differences.
  • Abundant genomic data provides a foundation for characterizing molecular pathways in nephron function.

Purpose of the Study:

  • To review advanced gene expression techniques for analyzing kidney nephron heterogeneity.
  • To discuss the potential of these techniques in understanding renal disease diagnostics.

Main Methods:

  • Quantitative reverse transcription PCR (RT-PCR) for analyzing specific messenger RNAs (mRNAs) from single microdissected renal cells.
  • Large-scale profiling techniques for a global view of gene expression regulation.

Main Results:

Related Experiment Videos

  • Sensitive gene expression techniques enable comprehensive molecular analysis of specific renal microenvironments.
  • These methods facilitate the study of functional differences across distinct nephron segments.

Conclusions:

  • Advanced molecular techniques offer powerful tools for characterizing nephron heterogeneity.
  • These approaches hold significant potential for diagnostic applications in renal diseases.