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Immunohistochemical labeling of normal melanocytes
Nicola R Dean1, James Brennan, Julie Haynes
1Co-operative Research Centre for Tissue Growth and Repair, Child Health Research Institute, Department of Plastic and Reconstructive Surgery, Royal Adelaide Hospital and University of Adelaide, Adelaide, Australia.
Abstract:
Comparison of seven antibodies for the demonstration of normal melanocytes in formalin-fixed, paraffin-embedded surgical discard skin showed that the monoclonal antibody Mel-5 (clone TA99) directed against pigment associated antigen was the most sensitive. Quantitative data were obtained for the sensitivity of the antibodies NKI/beteb, S100, T311, Melan A (clone A103), c-kit, and Mel-5 in parallel sections of human skin. An anticytokeratin antibody (CK34betaE12) was also used to stain basal keratinocytes and provide a negative image of the melanocytes present. Optimal conditions for the use of Mel-5 in paraffin sections of skin are described.