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Related Experiment Videos

A simple, low-cost, remote fiber-optic micro volume fluorescence flowcell for capillary flow-injection analysis.

Sean J Hart1, Renee D Jiji

  • 1Naval Research Laboratory, Chemistry Division, Biological Chemistry Section, Code 6113, 4555 Overlook Ave, S.W., Washington, DC 20375, USA. shart@ccf.nrl.navy.mil

Analytical and Bioanalytical Chemistry
|October 10, 2002
PubMed
Summary

Researchers developed a low-cost, small-volume flowcell for capillary flow injection (CFI) analysis using a commercial fluidic device. This innovative setup enables sensitive fluorescence detection with optical fibers and various light sources.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Microfluidics

Background:

  • Capillary flow injection (CFI) analysis requires sensitive detection methods.
  • Miniaturized flowcells are crucial for reducing sample consumption and analysis time.
  • Integrating optical detection with microfluidic devices presents engineering challenges.

Purpose of the Study:

  • To design and fabricate a low-cost, small-volume flowcell for fluorescence detection in CFI analysis.
  • To evaluate the performance of the flowcell using different excitation sources.
  • To demonstrate the utility of the flowcell for sensitive detection of fluorescent analytes.

Main Methods:

  • A commercially available fluidic cross device was adapted as a microflowcell with a swept volume of 721 nL.

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  • Optical fibers were employed for excitation light delivery and fluorescence emission collection, positioned at right angles.
  • Multiple excitation sources, including a UV laser and UV/blue LEDs, were utilized.
  • Detection limits were determined for fluorescein and bovine serum albumin (BSA).

    • Main Results:

    • The flowcell achieved sensitive fluorescence detection limits: 0.30 ppb for fluorescein and 2.1 x 10(-4)% (w/w) for BSA using a UV laser.
    • The system demonstrated flexibility with multiple excitation sources, including LEDs.
    • Two fluidic crosses in series enabled multi-wavelength fluorescence excitation with fiber-optically coupled LEDs.

    Conclusions:

    • A cost-effective and compact flowcell for CFI fluorescence detection was successfully developed.
    • The flowcell design facilitates sensitive and versatile fluorescence measurements.
    • This approach offers a practical solution for miniaturized analytical systems requiring fluorescence detection.