Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Subcellular mass determination by 4He+ energy-loss micro-spectrometry.

Guillaume Devès1, Richard Ortega

  • 1Chimie Nucléaire Analytrique et Bioenvironnementale CNRS UMRS084, Université de Bordeaux 1, BP 120 Le Haut-Vigneau, 33175 Gradignan cedex, France.

Analytical and Bioanalytical Chemistry
|October 10, 2002
PubMed
Summary

Scanning transmission ion microscopy (STIM) accurately measures intracellular mass in human cells. This technique, using a 4He+ microbeam, offers precise mass determination for biological samples, aiding in elemental analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metal Neurotoxicity assessment: A new imaging and analysis pipeline for primary neurons in co-cultures.

Journal of neuroscience methods·2026
Same author

Synchrotron XRF Imaging Reveals Manganese Accumulation in the Golgi and Post-Synapses of Neurons and Enhanced Uptake in Astrocytes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Storage and transport of labile iron is mediated by lysosomes in axons and dendrites of hippocampal neurons.

Metallomics : integrated biometal science·2025
Same author

Parkinson-like wild-type superoxide dismutase 1 pathology induces nigral dopamine neuron degeneration in a novel murine model.

Acta neuropathologica·2025
Same author

Synchrotron-based correlative imaging of metals and proteins in neuronal cells: state of the art and future challenges in neurometallomics.

Metallomics : integrated biometal science·2025
Same author

Native Cryo-Correlative Light and Synchrotron X-ray Fluorescence Imaging of Proteins and Essential Metals in Subcellular Neuronal Compartments.

Chemical & biomedical imaging·2024

Area of Science:

  • Biophysics
  • Cell Biology
  • Analytical Chemistry

Background:

  • Accurate intracellular mass determination is crucial for understanding cell physiology and pathology.
  • Existing methods may lack the precision or resolution required for detailed cellular analysis.

Purpose of the Study:

  • To establish and validate the Scanning Transmission Ion Microscope (STIM) as a precise method for quantifying intracellular mass in human cultured cells.
  • To demonstrate the utility of STIM in conjunction with particle-induced X-ray emission microanalysis (micro-PIXE) for elemental mapping.

Main Methods:

  • Utilized a 2.0 MeV 4He+ microbeam for enhanced ion-energy-loss sensitivity in freeze-dried, micron-thick human cells.
  • Employed dedicated software for local sample mass calculation based on energy-loss conversion and matrix stopping powers.

Related Experiment Videos

  • Validated the STIM method using epoxy resin sections and polymer foils (150-3000 nm thickness) with <5% error.

    • Main Results:

    • Achieved less than 5% error in mass determination during STIM analysis.
    • Determined a mean areal mass of 120 µg cm⁻² in freeze-dried human ovarian cancer cells.
    • Observed higher mass densities in the nucleus (200 µg cm⁻²) and nucleoli (250 µg cm⁻²).

    Conclusions:

    • STIM provides a highly accurate and reliable method for determining intracellular mass.
    • The combination of STIM and micro-PIXE enables true elemental concentration mapping within single cells.
    • This integrated approach is valuable for quantitative cell analysis and understanding cellular composition.